Application

Anti-MR1, clone 26.5, Cat. No. MABF2075, is a mouse monoclonal antibody that detects MHC class I-related gene protein and has been tested for use in Flow Cytometry, Function Analysis, and Immunoprecipitation.

Research CategoryInflammation & Immunology

Immunoprecipitation Analysis: A representative lot immunoprecipitated MR1 in Immunoprecipitation applications (Huang, S., et. al. (2005). J Biol Chem. 280(22):21183-93).

Flow Cytometry Analysis: A representative lot detected MR1 in flow cytometry applications (Huang, S., et. al. (2005). J Biol Chem. 280(22):21183-93; Gold, M.C., et. al. (2010). PLoS Biol. 8(6):e1000407; Huang, S., et. al. (2009). Proc Natl Acad Sci U S A. 106(20):8290-5).


Inhibits Activity/Function Analysis: A representative lot inhibited the activation of mucosal-associated invariant T (MAIT) cells by MR1 protein. (Huang, S., et. al. (2009). Proc Natl Acad Sci U S A. 106(20):8290-5).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Major histocompatibility complex class I-related gene protein (UniProt: Q95460; also known as MHC class I-related gene protein, Class I histocompatibility antigen-like protein, MR1) is encoded by the MR1 gene (Gene ID: 3140) in human. MR1 is a single-pass membrane protein that is ubiquitous in its distribution and can heterodimerize with B2M. It serves as an antigen-presenting molecule specialized in presenting microbial vitamin B metabolites. It is involved in the development and expansion of a small population of T-cells expressing an invariant T-cell receptor alpha chain known as mucosal-associated invariant T-cells (MAIT). MAIT lymphocytes are preferentially located in the gut lamina propria and may be involved in monitoring commensal flora or serve as a distress signal. MR1-restricted T cells can discriminate between microbes in a TCR-dependent manner. However, these cells are shown to be severely reduced in circulation in subjects with chronic HIV-1 infection. MR1 is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off in the mature form. The mature form has an extracellular domain (aa 23-302), a transmembrane domain (aa-303-323), and a short cytoplasmic tail (aa 324-341). Its ligand binding region is localized to amino acids 23-201. Five different isoforms of MR1 have been described that are produced by alternative splicing. (Ref.: Young, MH et al., (2013). PLOS one 8(1); e53789; Leeansyah, E., et al. (2013). Blood. 121(7); 1124-1135).

Immunogen

Epitope: extracellular domain

GST-tagged recombinant fragment corresponding to 280 amino acids from the extracellular domain of human MHC class I-related gene protein.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG2a in PBS without azide.

Format: Purified

Quality

Evaluated by Flow Cytometry in MR1-transfected WT3 mouse embryonic fibroblasts.

Flow Cytometry Analysis: 1 µg of this antibody detected MR1 in one million MR1-transfected WT3 mouse embryonic fibroblasts.

Specificity

Clone 26.5 specifically detects Major histocompatibility complex class I-related gene protein (MR1). It targets an epitope with in the extracellular domain.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

39.37 kDa calculated.